development of antibody-based microarray assay for quantitative detection of aflatoxin b1

background: aflatoxin b1 (afb1) is a toxic metaboliteproduced by aspergillus species that contaminates a wide range ofagricultural products. objectives: this study was designed todevelop a rapid and highly sensitive immunoassay method inmicroarray format for quantitative detection of afb1 to evaluatethe potential of microarray platform for high-throughput screening,which can be beneficial in food and feed industry. methods:following successful optimization, using an indirect competitiveimmunoassay in dot blot format, afb1-bovine serum albumin(afb1-bsa) conjugate was contact-printed onto 16 isolated subarrayson multi-pad nitrocellulose coated slides; subsequently usedin competitive binding assays. results: using the aforementionedassay, afb1 was determined from 15 pg/g to 3.04 ng/g workingrange with detection limit (lod) of 1 pg/g. to evaluate assayperformance in real food matrices, the authors spiked wheatsamples with different concentration of afb1. after extraction,working ranges of 0.11-4.15 ng/g with detection limit of 30pg/g wasdetermined. good recoveries (94±9%) were obtained, demonstratingaccurate detection of afb1 concentrations in wheat samples.assay procedure completed in 3 hours. conclusions: the resultsindicated that the proposed developed assay in microarray formatcould be used for rapid and sensitive detection of afb1in wheatsamples.